Determination of Oxalate in Urine (semi) automatic method. Suitable for all analyzers
Product code :
DETERMINATION OF OXALATE IN URINE (SEMI) AUTOMATIC METHOD
Oxalate is precipitated with calcium sulfate and ethanol, the precipitate is redissolved,
and the oxalate is oxidized to hydrogen peroxide and carbon dioxide by oxalate oxidase.
The hydrogen peroxide reacts with 3-Methyl-2-benzothiazolinone hydrazone (MBTH) and
diethylaniline (DEA) in the presence of peroxidase to yield an indamine dye with a maximum
absorbance at 590 nm.
Collect 24 h urine specimens in containers with 10 ml of 6 molar HCl.
Adjust the sample to pH 7.0 (± 0.5) prior for use.
Up to 1500 µmol/l Oxalate
Males: 80 - 490 μmol/24h
Females: 40 - 320 μmol/24h
Children: 140 - 420 μmol/24h
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